His first appearance on television was in the children's programme Tofsim Rosh in the early 1990s. Before he enlisted in the Israeli army at the age of 18, he participated in several Israeli TV shows, amongst them the series Lo Kolel Sherot and Inyan Shel Zman. After his army service he studied acting at Nissan Nativ's studio in Tel Aviv. During the course of his studying he participated in several student movies and also participated in the Israeli movies Girush Le Gan Eden (2003), Tgoova Meuheret (2004) and Offside (2006). In addition, he performed at the Gesher Theatre.
Between 2006-2007, he participated in the third and fourth seasons of the Israeli daily comedy musical drama HaShir Shelanu. In 2008, he participated in the American movie You Don't Mess with the Zohan alongside Adam Sandler.
Throughout the years, Mosseri participated also in dubbing several Animated series and movies to Hebrew, amongst them SpongeBob SquarePants, Cardcaptor Sakura, Jackie Chan Adventures, Pokemon, Teenage Mutant Ninja Turtles, TMNT, Treasure Planet, Sonic X, Transformers: Armada, Kung Fu Panda and Madagascar.
Ido is also the younger brother of popular Israeli entertainment personality Tal Mosseri.